Community articles — Reports

Artigo de pesquisa

Report on Modern Philosophy

Bacterial cells can have DNA damage due to transcriptional error, or through the effect of an antibiotic. The SOS response is a bacterial cell program for coping with DNA damage, in which the cell cycle is arrested, and DNA repair is induced. The repairs have high probability in leading to mutagenesis in the bacteria, which can lead to antibiotic resistance. The RecA protein in bacteria is responsible for the activation of the SOS response; therefore, making it a target for inhibition. I elected to use the ubiquitination system, natively used for apoptosis, as a means of targeted degradation of the RecA protein in bacteria prone to mutations. Polyubiquitination of misfolded proteins leads to the breaking down of the protein with the aid of proteasomes, which break down unnecessary proteins through a chemical reaction known as proteolysis. Using random forest-predictors, I determined a statistically high likelihood of ubiquitination of the RecA protein in MRSA, Tuberculosis, and other high risk bacterial infections. I hypothesized that I could foster ubiquitin-tagging on RecA by forcing the protein to misfold. Chaperones are proteins which interact with each other to prevent specific sets of proteins from misfolding. CHIP (C terminus of HSC70-Interacting Protein) is a biomolecule that inhibits interactions between the chaperones of RecA. Adding CHIP, ubiquitin, and 26s proteasomes into the bacterial system, should theoretically lead to the degradation of the RecA protein inside the bacteria. I tested my hypothesis by conducting an assay for monitoring CHIP-mediated ubiquitination, and conducted analysis on the assay using SDS- Page gel electrophoresis, and Western-blotting. The resulting data showed signs of polyubiquitination on the RecA protein, with chains of five or more ubiquitin, showing high drug potential. Adding an antibody drug conjugate, containing all the necessary components of a CHIP-mediated ubiquitination reaction, to common antibiotics can lead to the inhibition of bacterial mutagenesis, and higher antibiotic drug potency.

An experiment investigating full wave rectification, for the purposes of producing a steady DC output. This is implemented using every half-cycle input from the supply voltage instead of every other half, this is known as a full wave rectifier.

El análisis de sentimientos o minería opinión es el campo de estudio que analiza las opiniones, sentimientos, valoraciones, actitudes y emociones de la gente en lenguaje escrito. Es un área donde el procesamiento del lenguaje natural, la minería de datos, minería web y minería de texto se encuentran. Por ello, presentamos algunos conceptos necesarios para la comprensión de las características e implementación de estos métodos para la concepción de un sistema de un sistema de análisis de sentimientos.

Design and Implementation of distributed and fault-tolerant cellular core network

TEP4160 lab report

This report provides insight into the magnetic phenomenon of Hysteresis. Hysteresis is defined as a retardation effect where the magnetisation of a magnetic material lags behind the magnetizing force. Here we will explore the hysteresis loop for a silver steel ferromagnet and use this to discover it’s magnetic properties. The method used will be to place a ferromagnet inside a solenoid with an alternating voltage which will continually reverse the magnetic field and magnetism direction. The relation between these two quantities will be used to produce a hysteresis loop from which magnetic properties can be deduced. The results obtained were: saturation magnetisation = (8.4±0.5)(105)Am-1; remnant magnetisation = (5.9±0.5)(105)Am-1; coercive field: (4.3±0.5)(104)Am-1; energy expended per cycle per unit volume of material: (1.55±0.05)(103)Jm-3s-1; energy product: (8.7±3.0)(104)Jm-3.

目的： 熟悉分析實驗數據的方法，測量並分析誤差。 學習有效數的處理。 了解螺旋測微器(Micrometer)的操作方式並用以測定微小物體的厚度或直徑等。 了解游標尺(Vernier caliper)的操作方式並用以測量塑膠管的深度及內、外徑。